Composite
Part:BBa_K5089011
Designed by: Camille Prefontaine Group: iGEM24_UManitoba (2024-09-29)
ABO(H243R)-CsgA-LCI
This composite part consists of all components required for expressing and displaying ABO(H243R) and LCI on the cell surface of E.coli BL21(DE3). The characterization of the enzymatic activity of ABO(H243R) is available in the documentation for the base part BBa_K5089009. The characterization of LCI binding ability can be found in the documentation of the base part BBa_K3013000.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 812
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 831
Illegal NgoMIV site found at 977
Illegal AgeI site found at 335 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |